外泌体之家 | 细胞外膜泡领域核心平台—exosomes & microvesicles—小膜泡大作用
标题: What is a negative control for an exosome protein marker? [打印本页] 作者: luckkit 时间: 2015-4-10 12:45 标题: What is a negative control for an exosome protein marker? The International Society for Extracellular Vesicles recommends some negative controls, such as Grp94=HSP90B1, calnexin=CANX (both ER markers), GM130 (Golgi marker), Cytochrome C (mitochondrial marker), Histones (nuclear marker), and Argonaute =AGO (RISC complex marker). See this paper for details on the ISEV's recommendations for verifying that you have EVs: Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles.
J Extracell Vesicles. 2014 Dec 22;3:26913. doi: 10.3402/jev.v3.26913. eCollection 2014.