外泌体之家 | 细胞外膜泡领域核心平台—exosomes & microvesicles—小膜泡大作用
标题: 十年磨一剑，终于做完啦！！--投稿全记录 [打印本页] 作者: 惜名 时间: 2015-9-6 12:45 标题: 十年磨一剑，终于做完啦！！--投稿全记录 好吧，我承认我是标题党。。。
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鄙人实验室有2个人主要做exo，一个是师兄（其实和我一届，但年龄比我大很多），一个是我。
师兄平时要上临床，因此很忙，做的是DC-exo-Cardiomyocytes，未涉及很多机制，匆匆做了点现象，就投稿了。
被拒了。。。
其中一条拒稿意见是这样的：
The method of exosome isolation - ExoQuick is a well accepted method of exosome isolation. The yield is high, but the specificity is low. This paper (Lobb RJ Optimized exosome isolation protocol for cell culture supernatant and human plasma, J Extra Vesc, 2015) nicely sums up the problem: "In conclusion, both ExoQuickTM and Exo-spinTM have significantly more non-exosomal protein contamination as evidenced by the particle/protein ratio combined with increased exosome marker expression in qEV and density gradient isolations." This is borne out by your suspiciously high yield of protein from exosomes 10+ ug. Therefore, a number of non-exosomes may also be present in the tail-vein injections resulting in signaling from these activated dendritic cells. I encourage you to make note of this methodologic limitation in your conclusion.
用kit的童鞋们，都要小心啦。。。
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鄙人也基本做完了，正在整理数据写文章。
本着21世纪的“互联网分享精神”，打算在下次小聚时与大家分享我所有的结果。