外泌体之家 | 细胞外膜泡领域核心平台—exosomes & microvesicles—小膜泡大作用
标题: 求助文献全文 [打印本页] 作者: 篱下客 时间: 2016-2-13 16:55 标题: 求助文献全文 Lab Chip. 2016 Jan 26;16(3):489-96. doi: 10.1039/c5lc01117e. A microfluidic ExoSearch chip for multiplexed exosome detection towards blood-based ovarian cancer diagnosis.AbstractTumor-derived circulating exosomes, enriched with a group of tumor antigens, have been recognized as a promising biomarker source for cancer diagnosis via a less invasive procedure. Quantitatively pinpointing exosome tumor markers is appealing, yet challenging. In this study, we developed a simple microfluidic approach (ExoSearch) which provides enriched preparation of blood plasma exosomes for in situ, multiplexed detection using immunomagnetic beads. The ExoSearch chip offers a robust, continuous-flow design for quantitative isolation and release of blood plasma exosomes in a wide range of preparation volumes (10 μL to 10 mL). We employed the ExoSearch chip for blood-based diagnosis of ovarian cancer by multiplexed measurement of three exosomal tumor markers (CA-125, EpCAM, CD24) using a training set of ovarian cancer patient plasma, which showed significant diagnostic power (a.u.c. = 1.0, p = 0.001) and was comparable with the standard Bradford assay. This work provides an essentially needed platform for utilization of exosomes in clinical cancer diagnosis, as well as fundamental exosome research.
