circulating miRNA的主要存在形式

木瓜31

circulating miRNA的主要存在形式是Ago2-miRNA复合物还是囊泡相关？2011年PNAS的这篇文献“Argonaute2 complexes carry a population of
circulating microRNAs independent of vesicles in human plasma”认为是前者；2013发表在plos one的“The Major ity of MicroR NAs Detectable in Serum and saliva is concentrated in exosomes"认为是后者。现在有无较主流的说法？如果循环囊泡里的miRNA含量与non-vesicle circulating miRNA相比微乎其微，那其定量研究的意义何在？

大鹏子

hereissyk 发表于 2016-1-17 20:16
自己顶一下这个帖子，纠结中。
体液检测中直接测Circulating miRNAs与测exosome中miRNAs，两者有差别吗？
...

Characterization and deep sequencing analysis of exosomal and non-exosomal miRNA in human urine这篇文章里面写的 miRNAs were found to be significantly enriched and intact in urine-derived exosomes compared with cell-free urine ，这两者应该是有差别的吧

fjzmx

我也在困惑啊

limitzy

不懂啊，请大神出来

hereissyk

Several possibilities for the release of circulating miRNAs have been proposed as shown in Fig. 2: (1) passive leakage fromcells due to injury, chronic inflammation, or necrosis [6,7] including miR-208 [55], miR-122 [56], miR-192 [47], miR-21 [57], miR-200c [57], and miR-423 [57], which may probably release from the heart, liver and kidney due to injury, respectively. (2) Active secretion via membrane vesicles such as exosomes, L-exosome, microvesicles (MVs) and apoptotic bodies [58,59]. (3) Active secretion by complex formationwith lipoproteins (high-density lipoprotein, HDL) [60] and RNA binding proteins (AGO2 [61] and nucleophosmin 1 [62]).

Cheng, G. (2015). "Circulating miRNAs: roles in cancer diagnosis, prognosis and therapy." Adv Drug Deliv Rev 81: 75-93.

没有具体说明

hereissyk

另外，量与质是两个问题，就算exosome里面的miRNA总量少，但如果特异性高又何妨呢？

hereissyk

Plos One那篇文章的讨论有谈到两者为何不同，可能是方法不统一造成的，应该不用太纠结这个问题。

hereissyk

自己顶一下这个帖子，纠结中。
体液检测中直接测Circulating miRNAs与测exosome中miRNAs，两者有差别吗？

hereissyk

大鹏子 发表于 2016-1-18 19:46
Characterization and deep sequencing analysis of exosomal and non-exosomal miRNA in human urine这 ...

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