exosome的小鼠实验 我又要重新开始了

evomicscience

Your exosome should be labeled using fluorescence or luciferase or others. Otherwise, you will not know how much you dosage into mouse? By the way, protein alone (BAC) is not a reliable method to quantify exosome, specially if your exosome purity is not too high. Labeled exosome (engineering exosome) will help you to get ride of this issue and figure out where the dosage exosome is homing, in liver or tumor or spleen? In addition, how do you dosage exosome? hydrodynamic IV, IP and oral. the routine is issue for dosage. The cell type for exosome production is another issue too. In short, cell type ( immune cell or solid tumor cell), the dosage routine, and labeling exosome should be considerated.
Best, John