想统计下大家用超速离心时一次用多少量的细胞上清才够用

evomicscience

luckkit 发表于 2015-4-24 12:38
另外，请教一下，俺是收集上清后直接2000g 30min,然后0.22um过滤，10e4 30min, 10e5 2hrs, 开始没有300g 10 ...

the 1st 2000g may damage cell which then release miRNAs and/or proteins etc. You should use 300g to pellet cells,  and then 2000g for apoptotic bodies, 0.22um or 10k for microvesicle.