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本帖最后由 惜名 于 2016-7-4 09:35 编辑
本人正在投一篇Scientific Reports（水刊，求大神不要嘲笑）
用SBI试剂盒提取的exo，审稿人针对这个方法提出了如下comments，分享给各位群里的战友：
In Fig. 1, western blot analysis was performed using cell lysates and exosome lysates as starting materials. However, purified exosomes, especially if a polymer-based extraction kit such as the Exo-Quick kit is used, are heavily contaminated with carry-over proteins and cell debris from the initial DC culture media. Therefore, the authors should add a control were the same exosome samples are first digested with proteinase K to eliminate extra-exosomal contaminants and then lysed and used for western blot analysis. This control would help determine whether the indicated markers are of exosomal origin or not.
补实验中。。。都毕业了还要给自己擦屁股，唉。。。
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发表于 2016-7-4 09:34:01
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