What is a negative control for an exosome protein marker?

luckkit

The International Society for Extracellular Vesicles recommends some negative controls, such as Grp94=HSP90B1, calnexin=CANX (both ER markers), GM130 (Golgi marker), Cytochrome C (mitochondrial marker), Histones (nuclear marker), and Argonaute =AGO (RISC complex marker).  See this paper for details on the ISEV's recommendations for verifying that you have EVs:
Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles.
J Extracell Vesicles. 2014 Dec 22;3:26913. doi: 10.3402/jev.v3.26913. eCollection 2014.

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  http://www.ncbi.nlm.nih.gov/pubmed/25536934
  

ffp1988

thanks     越分享越快乐

小七果果8

谢谢啦~

张外泌体

正需要，谢谢

君扬2009

haowenzhang   xuexizhong